Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Triiodothyronine participates in odontoblast differentiation of apical papilla stem cells through regulation of ERK and p38MAPK signaling pathways

Jiali Xu, Lei He, Haibing Yang

Department of Stomatology, The Affiliated Changzhou No. 2 People's Hospital of Nanjing Medical University, Changzhou 213000, Jiangsu Province, China;

For correspondence:- Haibing Yang Email: yanghaibing2@163.com

Accepted: 25 August 2022 Published: 30 September 2022

Citation: Xu J, He L, Yang H. Triiodothyronine participates in odontoblast differentiation of apical papilla stem cells through regulation of ERK and p38MAPK signaling pathways. Trop J Pharm Res 2022; 21(9):1867-1872 doi: 10.4314/tjpr.v21i9.8

© 2022 The authors.
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Abstract

Purpose: To investigate the effect of triiodothyronine (T3) in odontoblast differentiation of apical papilla stem cells, and the mechanism of action involved.

Methods: Apical unclosed permanent molars extracted from patients due to orthodontics and impaction were selected. The extracted teeth were cultured in the isolation stage of SCAP cells. The cells were exposed to different concentrations of T3. The effects of ERK and p38 MAPK signaling pathways on activity of alkaline phosphatase (ALP) were determined. Calcium deposition was measured using a calcium determination kit, while the expression of BMP - 2 protein by T3 was determined by Western blot assay. Fluorescence quantitative polymerase chain reaction (FqPCR) method was used to determine the mRNA expression of BMP.

Results: The ALP activities were significantly higher in T3 groups than in control group. Relative to control, there were marked differences in ALP activity and calcium deposition in T3 group, T3 + PD group and T3 + SB group (p < 0.05). Relative to control, the mRNA and protein expressions of BMP-2 in T3 group were increased significantly (p < 0.05).

Conclusion: Triiodothyronine regulates the differentiation of apical papilla stem cells into dentin through ERK and p38MAPK signaling pathways. This provides the mechanism underlying odontoblast differentiation of apical papilla stem cells.

Keywords: Triiodothyronine, Differentiation, Apical papilla stem cells, Dentin, p38MAPK signal pathway, Phosphorylation